Figure 1. The saRNA samples were delivered into 293T cells using a transfection reagent at doses ranging from 0.781 to 12.5 ng per well. 48 hours post-transfection, firefly luciferase (FLuc) activity was measured by adding the D-luciferin substrate and recording luminescence with a microplate reader. The observed luminescence confirmed that FLuc protein was successfully expressed in the transfected cells.