EGFP Self-Amplifying RNA (saRNA) is engineered to enable sustained and amplified expression of an enhanced green fluorescent protein (EGFP). Derived from the jellyfish Aequorea victoria, EGFP retains its characteristic bright green fluorescence with excitation at 488 nm and emission at 509 nm, enabling direct fluorescence detection in live-cell imaging and long-term tracking applications. Unlike conventional mRNA, the saRNA leverages mRNA coding capacity with intrinsic self-replicating machinery for sustained EGFP expression.

• Positive Control for saRNA Transfection: Validating transfection efficiency and system performance.

• Delivery System Screening: Assessing nanoparticle/saRNA formulation efficacy in delivery applications.

• Based on VEEV self-replicating system with slight mutation to mitigate immunogenicity.

• A Cap1-AU structure with high capping efficiency.

• 100% substitution with 5-methylcytidine (m5C) for improved protein expression and reduced innate immune response

• A 100A poly(A) tail to mimic natural mature mRNA.

• Oligo-dT purification ensures high quality and purity.

• Carefully controlled processes to minimize batch-to-batch variation.