Fluc Self-Amplifying RNA (saRNA) is engineered to enable sustained and amplified expression of a firefly luciferase. Luciferase was originally extracted from the firefly (Photinus pyralis) and can catalyze the oxidation of luciferin to produce oxyluciferin, emitting yellow-green bioluminescence (wavelength of 550-570 nm) whose intensity directly reflects the expression level of luciferase. Unlike conventional mRNA, the saRNA leverages mRNA coding capacity with intrinsic self-replicating machinery for sustained luciferase expression.

• Positive Control for saRNA Transfection: Validating transfection efficiency and system performance.

• Delivery System Screening: Assessing nanoparticle/saRNA formulation efficacy in delivery applications.

• Based on VEEV self-replicating system with slight mutation to mitigate immunogenicity.

• A Cap1-AU structure with high capping efficiency.

• 100% substitution with 5-methylcytidine (m5C) for improved protein expression and reduced innate immune response.

• A 100A poly(A) tail to mimic natural mature mRNA.

• Oligo-dT purification ensures high quality and purity.

• Carefully controlled processes to minimize batch-to-batch variation.