Tdtomato mRNA is a red fluorescent protein encoded mRNA sequence derived from the Discosomacoral, engineered to express the tdTomato protein in cells after transfection. Originally isolated from the red fluorescent protein DsRed, tdTomato forms a stable intramolecular dimer through two tandem copies of the fluorescent moiety, exhibiting enhanced brightness and photostability compared to single-chain variants. It emits intense red fluorescence with excitation and emission maxima at 554 nm and 581 nm, respectively, enabling high-contrast imaging in live cells and tissues with minimal autofluorescence interference.
• Positive Control for mRNA Transfection: Validating transfection efficiency and system performance.
• Delivery System Screening: Assessing nanoparticle/mRNA formulation efficacy in delivery applications.
• Sequence optimized and selected for high expression.
• Oligo-dT purification ensures high quality and purity.
• Carefully controlled processes to minimize batch-to-batch variation.
1684 nt
2 mg/mL
1 mM sodium citrate, pH 6.5
Below -65℃
Dry ice
Visual
USP<791>
UV
UV
HPLC
CE
LC-MS
LC-MS
Nano Orange
ELISA
qPCR
Gel-clot method

Figure 1. tdTomato mRNA transfected into 293T cells.