EGFP-cy3 mRNA emits orange fluorescence with maximum excitation at ~550 nm and emission at ~570 nm. It also enables the expression of a red fluorescent protein EGFP in cells, organoids, or in vivo, with maximum excitation at ~488 nm and emission at ~509 nm. The Cy3 fluorescence can be utilized to track and localize the mRNA, while the detection of EGFP fluorescence serves to validate mRNA translation efficiency.

• Positive Control for mRNA Transfection: Validating transfection efficiency and system performance.

• Delivery System Screening: Assessing nanoparticle/mRNA formulation efficacy in delivery applications.

• Sequence optimized and selected for high expression.

• Oligo-dT purification ensures high quality and purity.

• Carefully controlled processes to minimize batch-to-batch variation.