EGFP mRNA is an enhanced green fluorescent protein (EGFP) encoded mRNA sequence, which can express EGFP protein in cells, organoids, or in vivo. EGFP was originally derived from the jellyfish Aequorea victoria, is a widely used reporter gene for direct fluorescence detection in cell culture and in vivo imaging. It produces bright green fluorescence with maximum excitation at 488 nm and emission at 509 nm.
• Positive Control for mRNA Transfection: Validating transfection efficiency and system performance.
• Delivery System Screening: Assessing nanoparticle/mRNA formulation efficacy in delivery applications.
• Sequence optimized and selected for high expression.
• Oligo-dT purification ensures high quality and purity.
• Carefully controlled processes to minimize batch-to-batch variation.
980 nt
1 mM sodium citrate, pH 6.5
Below -65℃
Dry ice
Visual
USP<791>
UV
UV
HPLC
CE
LC-MS
Nano Orange
ELISA
qPCR
Gel-clot method

Figure 1. RFU quantitative results of EGFP expression in HeLa cell at 48h after cell transfection.

Figure 2.Fluorescence photograph of EGFP expression results in four different cell types at 48 h after cell transfection with CatPure™ EGFP mRNA.

Figure 3. mRNA cell-free translation results by SDS-page.